In poor countries like Nepal we still depend upon Mantoux test besides clinical examinations, chest xray and sputum examination for diagnosis of Pulmonary Tuberculosis. In case of Sputum negative samples, we then send Sputum forGeneXpert to diagnose the case. What other investigational tools can precisely help in diagnosis of PTB? What are the latest diagnostic investigations available? Are they feasible and readily available in country like ours?