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Field partner for testing assay to detect Wolbachia in mosquitos?

By Jacqueline Linnes | 04 May, 2016

I am posting on behalf of a colleague at the University of Texas, Austin who has developed a test based on isothermal nucleic acid amplification (LAMP-based I believe) to detect Wolbachia and in mosquitoes. In the lab, they can perform assays with crushed mosquitoes, and return sensitive detection with a minimalist device in ~30 minutes. They would like to find field partners to partner with on an upcoming grant proposal who could test the usability and applicability of the device for detection from mosquitoes in the field. Please let me know and I can work to connect you.



Robert Vermaire Replied at 5:39 PM, 4 May 2016

Excuse me for my ignorance, but is this a study simply to find the prevalence of Wolbachia in various geographic regions? What is the next step in regards to countering malaria?

Tony Kiszewski Replied at 3:45 AM, 5 May 2016

I'm definitely interested. I do extensive surveillance of tree hole and container breeding mosquitoes in the Boston area and was interested in characterizing their Wohlbachia prevalence.

Jennifer Stevenson Replied at 4:08 AM, 5 May 2016

Hi Jacqueline

We at Macha Research Trust in collaboration with Johns Hopkins School of
Public Health have extensive experience of working on mosquitoes
specifically anophelines in Zambia. We have well established insectaries, a
molecular laboratory where we have evaluated LAMP assays previously,
skilled staff and very productive field teams here in southern Zambia. We
also have a newly established semi field system in which we are studying
bacterial infections of mosquitoes so we are well placed to study Wolbachia.

Do get in contact if working with us would be of interest.

Scientific Director and Resident entomologist, Macha Research Trust, Zambia
Research Associate, Johns Hopkins Malaria Research Institute, USA

Bamou Roland Replied at 6:00 AM, 5 May 2016

Hello i am young student from Cameroon working in entomology and other vector borne disease.I will be good if we can be part of this proposal.
we are wirking with reseaecher from Oceac and molecular biologist trained at icipe, Kenya.
Identification if this endosymbiote in central region and cameroon could ne done by at
university of dschang at molecular parasitology unit.

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